Species:

Rat

Alternative Name:

Amine oxidase [flavin containing] A,AOFA,EC 1.4.3.4,MAO A,MAO-A,maoA,Monoamine oxidase A,Monoamine oxidase type A

Uniport ID:

P21396

Assay Type:

Sandwich

unit:

ng/mL

Standard:

10ng/mL

Sensitivity:

0.1 ng/mL

Detection Range:

0.16-10ng/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Research:

CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MAOA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAOA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MAOA.You can calculate the concentration of Rat MAOA in the samples by comparing the OD of the samples to the standard curve.